Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28143-1-AP targets DPP6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27338 Product name: Recombinant human DPP6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 377-596 aa of BC150304 Sequence: KDGRKFFFIRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITSGDWDVTKILAYDEKGNKIYFLSTEDLPRRRQLYSANTVGNFNRQCLSCDLVENCTYFSASFSHSMDFFLLKCEGPGVPMVTVHNTTDKKKMFDLETNEHVKKAINDRQMPKVEYRDIEIDDYNLPMQILKPATFTDTTHYPLLLVVDGTPGSQSVAEKFEVSWETVMVSSHGAVVVKC 相同性解析による交差性が予測される生物種 |
| Full Name | dipeptidyl-peptidase 6 |
| Observed molecular weight | 98-101 kDa |
| GenBank accession number | BC150304 |
| Gene Symbol | DPP6 |
| Gene ID (NCBI) | 1804 |
| RRID | AB_3669647 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42658 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DPP6 (Dipeptidyl aminopeptidase-like protein 6) is also named as DPPX and Dipeptidyl peptidase VI (DPP VI). DPP6 is a type II transmembrane protein, and DPP6 is a multifunctional protein with considerable impact on brain development and function (PMID: 35395074). In breast cancer tissues, DPP6 has low expression at the transcription and protein levels, and DPP6 may serve as a tumour suppressor in tumour development (PMID: 36531033).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DPP6 antibody 28143-1-AP | Download protocol |
| IP protocol for DPP6 antibody 28143-1-AP | Download protocol |
| WB protocol for DPP6 antibody 28143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |