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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP4/CD26 Recombinant antibody (84606-6-RR)

    WB analysis using 84606-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using DPP4/CD26 Recombinant antibody (84606-6-RR)

    WB analysis of human placenta using 84606-6-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DPP4/CD26 Recombinant antibody (84606-6-RR)

    IHC staining of human prostate cancer using 84606-6-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DPP4/CD26 Recombinant antibody (84606-6-RR)

    IHC staining of human prostate cancer using 84606-6-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using DPP4/CD26 Recombinant antibody (84606-6-RR)

    IF Staining of HuH-7 using 84606-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HuH-7 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DPP4/CD26 Recombinant antibody (84606-6-RR)

    IF Staining of PC-3 using 84606-6-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84606-6-RR

    Affinity and Kinetic Characterization of 84606-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84606-6-RR against Human DPP4/CD26 were performed. The affinity constant is 1.59 nM.

DPP4/CD26 Recombinant monoclonal antibody

DPP4/CD26 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84606-6-RR
Clone No.242045F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD26, 242045F5, ADABP, ADCP 2, ADCP2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, human placenta tissue, LNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHuH-7 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84606-6-RR targets DPP4/CD26 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0157

Product name: Recombinant Human DPP4/CD26 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 34-766 aa of BC013329

Sequence: DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight110 kDa
GenBank accession numberBC013329
Gene Symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP27487
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

Protocols

Product Specific Protocols
IF protocol for DPP4/CD26 antibody 84606-6-RRDownload protocol
IHC protocol for DPP4/CD26 antibody 84606-6-RRDownload protocol
WB protocol for DPP4/CD26 antibody 84606-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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