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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP4/CD26 Polyclonal antibody (32397-1-AP)

    WB analysis using 32397-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using DPP4/CD26 Polyclonal antibody (32397-1-AP)

    IHC staining of rat liver using 32397-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DPP4/CD26 Polyclonal antibody (32397-1-AP)

    IHC staining of mouse liver using 32397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DPP4/CD26 Polyclonal antibody (32397-1-AP)

    IHC staining of mouse liver using 32397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using DPP4/CD26 Polyclonal antibody (32397-1-AP)

    IHC staining of rat liver using 32397-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DPP4/CD26 Polyclonal antibody

DPP4/CD26 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32397-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, rat lung tissue
Positive IHC detected inrat liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32397-1-AP targets DPP4/CD26 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name dipeptidylpeptidase 4
Calculated molecular weight87 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_010074.3
Gene Symbol Dpp4
Gene ID (NCBI) 13482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP28843
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).

Protocols

Product Specific Protocols
WB protocol for DPP4/CD26 antibody 32397-1-APDownload protocol
IHC protocol for DPP4/CD26 antibody 32397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols