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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP10 Polyclonal antibody (12553-1-AP)

    WB analysis using 12553-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12553-1-AP (DPP10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DPP10 Polyclonal antibody (12553-1-AP)

    WB analysis using 12553-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12553-1-AP (DPP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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DPP10 Polyclonal antibody

DPP10 Polyclonal Antibody for WB, ELISA
Cat No. 12553-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2, DPL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12553-1-AP targets DPP10 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP10 fusion protein Ag3231 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 10
Calculated molecular weight 796 aa, 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC030832
Gene Symbol DPP10
Gene ID (NCBI) 57628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N608
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DPP10 (dipeptidyl aminopeptidase-like protein 10) is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels (PMID: 30638748). DPP10 is prominently expressed in the brain, notably present in neuronal populations (PMID: 15671030).

Protocols

Product Specific Protocols
WB protocol for DPP10 antibody 12553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols