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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DPM1 Polyclonal antibody (12403-2-AP)

    WB analysis of HeLa using 12403-2-AP (same clone as 12403-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12403-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DPM1 Polyclonal antibody (12403-2-AP)

    WB analysis of HepG2 using 12403-2-AP (same clone as 12403-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12403-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DPM1 Polyclonal antibody (12403-2-AP)

    IHC staining of human liver cancer using 12403-2-AP (same clone as 12403-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12403-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DPM1 Polyclonal antibody (12403-2-AP)

    IHC staining of human liver cancer using 12403-2-AP (same clone as 12403-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12403-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DPM1 Polyclonal antibody, PBS Only

DPM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12403-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

DPM synthase subunit 1, DPM synthase, Dolichyl-phosphate beta-D-mannosyltransferase subunit 1, Dolichol-phosphate mannosyltransferase subunit 1, Dolichol-phosphate mannose synthase subunit 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12403-2-PBS targets DPM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1128

Product name: Recombinant human DPM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC007073

Sequence: MASLEVSRSPRRSRRELEVRSPRQNKYSVLLPTYNERENLPLIVWLLVKSFSESGINYEIIIIDDGSPDGTRDVAEQLEKIYGSDRILLRPREKKLGLGTAYIHGMKHATGNYIIIMDADLSHHPKFIPEFIRKQKEGNFDIVSGTRYKGNGGVYGWDLKRKIISRGANFLTQILLRPGASDLTGSFRLYRKEVLEKLIEKCVSKGYVFQMEMIVRARQLNYTIGEVPISFVDRVYGESKLGGNEIVSFLKGLLTLFATT

相同性解析による交差性が予測される生物種
Full Name dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
Calculated molecular weight 260 aa, 30 kDa
Observed molecular weight 27-32 kDa
GenBank accession numberBC007073
Gene Symbol DPM1
Gene ID (NCBI) 8813
RRIDAB_2093270
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60762
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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