Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14866-1-AP targets DPH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DPH2 fusion protein Ag6678 相同性解析による交差性が予測される生物種 |
| Full Name | DPH2 homolog (S. cerevisiae) |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC001389 |
| Gene Symbol | DPH2 |
| Gene ID (NCBI) | 1802 |
| RRID | AB_2093254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DPH2 antibody 14866-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |