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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using DPH2 Polyclonal antibody (12367-1-AP)

    IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

    IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DPH2 Polyclonal antibody (12367-1-AP)

    IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DPH2 Polyclonal antibody (12367-1-AP)

    IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

DPH2 Polyclonal antibody, PBS Only

DPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12367-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12367-1-PBS targets DPH2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3042

Product name: Recombinant human DPH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-489 aa of BC016956

Sequence: SPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG

相同性解析による交差性が予測される生物種
Full Name DPH2 homolog (S. cerevisiae)
Calculated molecular weight 489 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC016956
Gene Symbol DPH2
Gene ID (NCBI) 1802
RRIDAB_2093256
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQC3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

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