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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPF1 Polyclonal antibody

DPF1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21769-1-AP

Synonyms

BAF45B, BRG1 associated factor 45B, DPF1, NEUD4, neuro d4, Zinc finger protein neuro d4

Validation Data Gallery

Applications:
  • WB analysis using 21769-1-AP

    WB analysis using 21769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21769-1-AP (DPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21769-1-AP

    IF Staining of MCF-7 using 21769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DPF1 antibody (21769-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, PC-3 cells, mouse cerebellum tissue
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy DPF1 Ready-To-Use IHC Kit. DPF1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

21769-1-AP targets DPF1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPF1 fusion protein Ag16409 相同性解析による交差性が予測される生物種
Full Name D4, zinc and double PHD fingers family 1
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight38-47 kDa
GenBank accession numberBC125153
Gene symbol DPF1
Gene ID (NCBI) 8193
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPF1 antibody 21769-1-APDownload protocol
IF protocol for DPF1 antibody 21769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols