• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F4

Cat no : 68081-1-Ig

Synonyms

Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase

Validation Data Gallery

Applications:
  • WB analysis of pig pancreas using 68081-1-Ig

    WB analysis of pig pancreas using 68081-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 68081-1-Ig

    WB analysis of human testis using 68081-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit kidney using 68081-1-Ig

    WB analysis of rabbit kidney using 68081-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat pancreas using 68081-1-Ig

    WB analysis of rat pancreas using 68081-1-Ig

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68081-1-Ig

    WB analysis of HepG2 using 68081-1-Ig

    WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

  • IHC staining of human kidney using 68081-1-Ig

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68081-1-Ig

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 68081-1-Ig

    IF Staining of mouse kidney using 68081-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68081-1-Ig targets DPEP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DPEP1 fusion protein Ag14591 相同性解析による交差性が予測される生物種
Full Name dipeptidase 1 (renal)
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC017023
Gene symbol DPEP1
Gene ID (NCBI) 1800
RRIDAB_2918818
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPEP1 antibody 68081-1-IgDownload protocol
IHC protocol for DPEP1 antibody 68081-1-IgDownload protocol
IF protocol for DPEP1 antibody 68081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols