Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85811-2-RR targets DOPA decarboxylase/DDC in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | DOPA decarboxylase/DDC fusion protein Ag0219 相同性解析による交差性が予測される生物種 |
| Full Name | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC008366 |
| Gene Symbol | DOPA decarboxylase |
| Gene ID (NCBI) | 1644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOPA decarboxylase/DDC antibody 85811-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |