DOCK8 Recombinant antibody
DOCK8 Recombinant Antibody for WB, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
230441A2
Cat no : 83270-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Ramos cells, Raji cells, THP-1 cells |
Positive IF detected in | THP-1 cells |
Positive FC detected in | THP-1 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
83270-2-RR targets DOCK8 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | DOCK8 fusion protein Ag34585 相同性解析による交差性が予測される生物種 |
Full Name | dedicator of cytokinesis 8 |
Calculated molecular weight | 2031 aa, 231 kDa |
Observed molecular weight | 239 kDa |
GenBank accession number | BC019102 |
Gene symbol | DOCK8 |
Gene ID (NCBI) | 81704 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).
Protocols
Product Specific Protocols | |
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WB protocol for DOCK8 antibody 83270-2-RR | Download protocol |
IF protocol for DOCK8 antibody 83270-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |