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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK6 Polyclonal antibody (25087-1-AP)

    WB analysis using 25087-1-AP (same clone as 25087-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon using 25087-1-AP (same clone as 25087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon using 25087-1-AP (same clone as 25087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon cancer using 25087-1-AP (same clone as 25087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon cancer using 25087-1-AP (same clone as 25087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25087-1-PBS in a different storage buffer formulation.

DOCK6 Polyclonal antibody, PBS Only

DOCK6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25087-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25087-1-PBS targets DOCK6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18901

Product name: Recombinant human DOCK6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1699-2041 aa of BC146786

Sequence: LYEAVNEVYKNLIPILEAHRDYKKLAAVHGKLQEAFTKIMHQSSGWERVFGTYFRVGFYGAHFGDLDEQEFVYKEPSITKLAEISHRLEEFYTERFGDDVVEIIKDSNPVDKSKLDSQKAYIQITYVEPYFDTYELKDRVTYFDRNYGLRTFLFCTPFTPDGRAHGELPEQHKRKTLLSTDHAFPYIKTRIRVCHREETVLTPVEVAIEDMQKKTRELAFATEQDPPDAKMLQMVLQGSVGPTVNQGPLEVAQVFLAEIPEDPKLFRHHNKLRLCFKDFCKKCEDALRKNKALIGPDQKEYHRELERNYCRLREALQPLLTQRLPQLMAPTPPGLRNSLNRAS

相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 6
Calculated molecular weight 2047 aa, 230 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC146786
Gene Symbol DOCK6
Gene ID (NCBI) 57572
RRIDAB_2879889
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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