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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK5 Polyclonal antibody (29938-1-AP)

    WB analysis using 29938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DOCK5 Polyclonal antibody (29938-1-AP)

    WB analysis using 29938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DOCK5 Polyclonal antibody (29938-1-AP)

    WB analysis of A549 using 29938-1-AP

    WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using DOCK5 Polyclonal antibody (29938-1-AP)

    WB analysis using 29938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of DU 145 cells using DOCK5 Polyclonal antibody (29938-1-AP)

    IP experiment of DU 145 using 29938-1-AP

    IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DOCK5 Polyclonal antibody (29938-1-AP)

    IHC staining of mouse kidney using 29938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DOCK5 Polyclonal antibody (29938-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DOCK5 Polyclonal antibody (29938-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DOCK5 Polyclonal antibody (29938-1-AP)

    IF Staining of U2OS using 29938-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

DOCK5 Polyclonal antibody

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 29938-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

dedicator of cytokinesis 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, A549 cells, DU 145 cells, NIH3T3 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue
Positive IP detected inDU 145 cells
Positive IHC detected inmouse kidney tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29938-1-AP targets DOCK5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32193

Product name: Recombinant human DOCK5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1700-1870 aa of BC137175

Sequence: ILEPLLERRASSGARVEDLSLREENSENRISKFKRKDWSLSKSQVIAEKAPEPDLMSPTRKAQRPKSLQLMDNRLSPFHGSSPPQSTPLSPPPLTPKATRTLSSPSLQTDGIAATPVPPPPPPKSKPYEGSQRNSTELAPPLPVRREAKAPPPPPPKARKSGIPTSEPGSQ

相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 5
Calculated molecular weight 1870 aa, 215 kDa
Observed molecular weight190 kDa
GenBank accession numberBC137175
Gene Symbol DOCK5
Gene ID (NCBI) 80005
RRIDAB_3086194
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7D0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.

Protocols

Product Specific Protocols
IF protocol for DOCK5 antibody 29938-1-APDownload protocol
IHC protocol for DOCK5 antibody 29938-1-APDownload protocol
IP protocol for DOCK5 antibody 29938-1-APDownload protocol
WB protocol for DOCK5 antibody 29938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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