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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of Ramos using 66969-1-Ig (same clone as 66969-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DOCK2 Monoclonal antibody (66969-1-Ig)

    IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DOCK2 Monoclonal antibody (66969-1-Ig)

    IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DOCK2 Monoclonal antibody, PBS Only

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66969-1-PBS
Clone No.1D7A8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66969-1-PBS targets DOCK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28582

Product name: Recombinant human DOCK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-337 aa of BC016996

Sequence: MCVCAEDGKTLPVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGTTLHDGFHDLVVLKGDSKKMEDASAYLTLPSYRHHVENKGATLSRSSSSVGGLSVSSRDVFSISTLVCSTKLTQNVGLLGLLKWRMKPQLEESMRRLFESINNLMKSQYKTTILLQVAALKYIPSVLHDVEMVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMNEIVQSNLFKKQECRDILLPVITKELKELLEQKDDMQHQVLERKYCVELLNSILEVLSYQDAAFTYHHIQEIMVQLLRTVNRTVITMGRDHILIGACCNCGFPSSTPHSLIQWLWG

相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 2
Calculated molecular weight 212 kDa, 38 kDa
Observed molecular weight 200-210 kDa
GenBank accession numberBC016996
Gene Symbol DOCK2
Gene ID (NCBI) 1794
RRIDAB_2882291
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ92608
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

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