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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK11 Recombinant antibody (85022-3-RR)

    WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DOCK11 Recombinant antibody (85022-3-RR)

    WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DOCK11 Recombinant antibody (85022-3-RR)

    WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01759-2

    Cytometric bead array standard curve of MP01759-2

    Cytometric bead array standard curve of MP01759-2, DOCK11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85022-3-PBS. Detection antibody: 85022-1-PBS. Standard: Ag35831. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85022-3-RR

    Affinity and Kinetic Characterization of 85022-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85022-3-RR against Human DOCK11 were performed. The affinity constant is below 1 pM.

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DOCK11 Recombinant antibody, PBS Only (Capture)

DOCK11 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 85022-3-PBS
Clone No.242575H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

242575H5, ACG, bB128O4.1, dedicator of cytokinesis 11, ZIZ2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85022-3-PBS targets DOCK11 as part of a matched antibody pair:

MP01759-2: 85022-3-PBS capture and 85022-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DOCK11 fusion protein Ag35831 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 11
Calculated molecular weight238 kDa
Observed molecular weight238 kDa
GenBank accession numberNM_144658
Gene Symbol DOCK11
Gene ID (NCBI) 139818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5JSL3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.