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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK11 Polyclonal antibody (55412-1-AP)

    WB analysis using 55412-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55412-1-AP (DOCK11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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DOCK11 Polyclonal antibody

DOCK11 Polyclonal Antibody for WB, ELISA
Cat No. 55412-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Zizimin 2, ZIZ2, dedicator of cytokinesis 11, bB128O4.1, ACG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55412-1-AP targets DOCK11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 11
Calculated molecular weight 238 kDa
GenBank accession numberNM_144658
Gene Symbol DOCK11
Gene ID (NCBI) 139818
RRIDAB_3086449
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JSL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.

Protocols

Product Specific Protocols
WB protocol for DOCK11 antibody 55412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols