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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DOC2B Polyclonal antibody (20574-1-AP)

    WB analysis of HEK-293 using 20574-1-AP (same clone as 20574-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using DOC2B Polyclonal antibody (20574-1-AP)

    WB analysis of mouse brain using 20574-1-AP (same clone as 20574-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using DOC2B Polyclonal antibody (20574-1-AP)

    WB analysis of mouse kidney using 20574-1-AP (same clone as 20574-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20574-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DOC2B Polyclonal antibody (20574-1-AP)

    IF Staining of HepG2 using 20574-1-AP (same clone as 20574-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20574-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOC2B Polyclonal antibody (20574-1-AP)

    IF Staining of HeLa using 20574-1-AP (same clone as 20574-1-PBS)

    Immunofluorescent analysis of Hela cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20574-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DOC2B Polyclonal antibody, PBS Only

DOC2B Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20574-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20574-1-PBS targets DOC2B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name double C2-like domains, beta
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_003585
Gene Symbol DOC2B
Gene ID (NCBI) 8447
RRIDAB_10696316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14184
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.

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