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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNPEP Polyclonal antibody (15016-1-AP)

    WB analysis using 15016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DNPEP Polyclonal antibody (15016-1-AP)

    WB analysis of MCF-7 using 15016-1-AP

    WB result of DNPEP antibody (15016-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNPEP transfected MCF-7 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DNPEP Polyclonal antibody (15016-1-AP)

    IP experiment of HEK-293 using 15016-1-AP

    IP result of anti-DNPEP (IP:15016-1-AP, 3ug; Detection:15016-1-AP 1:500) with HEK-293 cells lysate 1760ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNPEP Polyclonal antibody (15016-1-AP)

    IHC staining of mouse testis using 15016-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15016-1-AP (DNPEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNPEP Polyclonal antibody (15016-1-AP)

    IHC staining of mouse testis using 15016-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15016-1-AP (DNPEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DNPEP Polyclonal antibody (15016-1-AP)

    IF Staining of HEK-293 using 15016-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DNPEP antibody (15016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

DNPEP Polyclonal antibody

DNPEP Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15016-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

EC:3.4.11.21, DAP, ASPEP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15016-1-AP targets DNPEP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6966

Product name: Recombinant human DNPEP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-475 aa of BC000653

Sequence: FDRDLTLAGRVIVKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINENFGPNTEMHLVPILATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHLGLSPKDIVEMELCLADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNAVSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAMHSIREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD

相同性解析による交差性が予測される生物種
Full Name aspartyl aminopeptidase
Calculated molecular weight 52 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000653
Gene Symbol DNPEP
Gene ID (NCBI) 23549
RRIDAB_2094293
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues.

Protocols

Product Specific Protocols
WB protocol for DNPEP antibody 15016-1-APDownload protocol
IF protocol for DNPEP antibody 15016-1-APDownload protocol
IP protocol for DNPEP antibody 15016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling.

Authors - Nakamura Yukio Y
View Article