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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT1 Recombinant antibody (82678-2-RR)

    WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNMT1 Recombinant antibody (82678-2-RR)

    FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82678-2-RR

    Affinity and Kinetic Characterization of 82678-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82678-2-RR against Human DNMT1 were performed. The affinity constant is below 1 pM.

DNMT1 Recombinant monoclonal antibody, PBS Only

DNMT1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82678-2-PBS
Clone No.240302E2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

240302E2, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82678-2-PBS targets DNMT1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29479

Product name: Recombinant human DNMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 300-500 aa of BC126227

Sequence: KKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFA

相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight 1632 aa, 185 kDa
GenBank accession numberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP26358
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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