Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, pig cerebellum tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
24362-1-AP targets DNER in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19955 Product name: Recombinant human DNER protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 75-387 aa of BC035009 Sequence: EPGYSCTCPAGISGANCQLVADPCASNPCHHGNCSSSSSSSSDGYLCICNEGYEGPNCEQALPSLPATGWTESMAPRQLQPVPATQEPDKILPRSQATVTLPTWQPKTGQKVVEMKWDQVEVIPDIACGNASSNSSAGGRLVSFEVPQNTSVKIRQDATASLILLWKVTATGFQQCSLIDGRSVTPLQASGGLVLLEEMLALGNNHFIGFVNDSVTKSIVALRLTLVVKVSTCVPGESHANDLECSGKGKCTTKPSEATFSCTCEEQYVGTFCEEYDACQRKPCQNNASCIDANEKQDGSNFTCVCLPGYTGE 相同性解析による交差性が予測される生物種 |
| Full Name | delta/notch-like EGF repeat containing |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 110-130 kDa |
| GenBank accession number | BC035009 |
| Gene Symbol | DNER |
| Gene ID (NCBI) | 92737 |
| RRID | AB_3085732 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Delta and Notch-like epidermal growth factor-related receptor (DNER) is a type I transmembrane protein that is specifically expressed in the developing and mature central nervous system (PMID: 11950833). It contains ten extracellular EGF-like repeats that are closely related to those of the developmentally important receptor Notch and its ligand Delta. DNER functions as a Notch ligand and mediates signaling through neuron-glia interactions (PMID: 15965470).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNER antibody 24362-1-AP | Download protocol |
| WB protocol for DNER antibody 24362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
