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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNASE1L1 Polyclonal antibody

DNASE1L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13653-1-AP

Synonyms

Deoxyribonuclease 1 like 1, deoxyribonuclease I like 1, DNAS1L1, DNase I like 1, DNase X, DNASE1L1, DNASEX, DNL1L, Muscle specific DNase I like, XIB

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 13653-1-AP

    WB analysis of mouse skeletal muscle using 13653-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat skeletal muscle using 13653-1-AP

    WB analysis of rat skeletal muscle using 13653-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 13653-1-AP

    IP experiment of mouse skeletal muscle using 13653-1-AP

    IP result of anti-DNASE1L1 (IP:13653-1-AP, 4ug; Detection:13653-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

  • IHC staining of human skeletal muscle using 13653-1-AP

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 13653-1-AP

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 13653-1-AP

    IF Staining of MCF-7 using 13653-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DNASE1L1 antibody 13653-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13653-1-AP targets DNASE1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE1L1 fusion protein Ag4222 相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease I-like 1
Calculated molecular weight 302 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC028092
Gene symbol DNASE1L1
Gene ID (NCBI) 1774
RRIDAB_2230813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IHC protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IF protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IP protocol for DNASE1L1 antibody 13653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cells

New Findings: Hindlimb Unloading Causes Nucleocytoplasmic Ca2+ Overload and DNA Damage in Skeletal Muscle

Authors - Huajian Yang
View Article
ratWB

J Tradit Chin Med

Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

Authors - Zhu Bin
  • KD Validated
View Article