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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNALI1 Recombinant antibody (85734-1-RR)

    WB analysis using 85734-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85734-1-RR (DNALI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse fallopian tube tissue using DNALI1 Recombinant antibody (85734-1-RR)

    IHC staining of mouse fallopian tube using 85734-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue slide using 85734-1-RR (DNALI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85734-1-RR

    Affinity and Kinetic Characterization of 85734-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85734-1-RR against Human DNALI1 were performed. The affinity constant is below 1 pM.

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DNALI1 Recombinant antibody

DNALI1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85734-1-RR
Clone No.243157E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

hp28, P28

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse fallopian tube tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNALI1 fusion protein Ag11652 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, light intermediate chain 1
Calculated molecular weight 258 aa, 30 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC046117
Gene Symbol DNALI1
Gene ID (NCBI) 7802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

Protocols

Product Specific Protocols
WB protocol for DNALI1 antibody 85734-1-RRDownload protocol
IHC protocol for DNALI1 antibody 85734-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols