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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    WB analysis using 21941-1-AP (same clone as 21941-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21941-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IP experiment of mouse brain using 21941-1-AP (same clone as 21941-1-PBS)

    IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 21941-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IHC staining of mouse brain using 21941-1-AP (same clone as 21941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21941-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IHC staining of mouse brain using 21941-1-AP (same clone as 21941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21941-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IF Staining of HeLa using 21941-1-AP (same clone as 21941-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC6/AUXILIN antibody (21941-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21941-1-PBS in a different storage buffer formulation.

DNAJC6/AUXILIN Polyclonal antibody, PBS Only

DNAJC6/AUXILIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21941-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Auxilin, DNAJC6, DJC6, EC:3.1.3.-, KIAA0473

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21941-1-PBS targets DNAJC6/AUXILIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16612

Product name: Recombinant human DNAJC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 387-733 aa of BC109279

Sequence: IPLDTTVLKFTKPELDACDVPEKYPQLFQVTLDVELQPHDKVIDLTPPWEHYCTKDVNPSILFSSHQEHQDTLALGGQAPIDIPPDNPRHYGQSGFFASLCWQDQKSEKSFCEEDHAALVNQESEQSDDELLTLSSPHGNANGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTPRRSATSTSASPTLRVGEGATFDPFGAPSKPSGQDLLGSFLNTSSASSDPFLQPTRSPSPTVHASSTPAVNIQPDVSGGWDWHAKPGGFGMGSKSAATSPTGSSHGTPTHQSKPQTL

相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 6
Calculated molecular weight 970 aa, 106 kDa
Observed molecular weight 100-110 kDa, 86 kDa
GenBank accession numberBC109279
Gene Symbol DNAJC6/AUXILIN
Gene ID (NCBI) 9829
RRIDAB_2878951
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75061
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

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