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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC3 Polyclonal antibody (26721-1-AP)

    WB analysis using 26721-1-AP (same clone as 26721-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26721-1-AP (DNAJC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of mouse testis using 26721-1-AP (same clone as 26721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of mouse testis using 26721-1-AP (same clone as 26721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DNAJC3 Polyclonal antibody (26721-1-AP)

    IF Staining of U2OS using 26721-1-AP (same clone as 26721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 26721-1-AP (DNAJC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

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DNAJC3 Polyclonal antibody, PBS Only

DNAJC3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 26721-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26721-1-PBS targets DNAJC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC3 fusion protein Ag24966 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 3
Observed molecular weight58 kDa
GenBank accession numberBC047936
Gene Symbol DNAJC3
Gene ID (NCBI) 5611
RRIDAB_2880613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13217
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).