Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31580-1-AP targets DNAJC17 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35992 Product name: Recombinant human DNAJC17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 69-194 aa of BC000048 Sequence: RAAYDKVRKAKKQAAERTQKLDEKRKKVKLDLEARERQAQAQESEEEEESRSTRTLEQEIERLREEGSRQLEEQQRLIREQIRQERDQRLRGKAENTEGQGTPKLKLKWKCKKEDESKGGYSKDVL 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 17 |
| Calculated molecular weight | 304 aa, 35 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000048 |
| Gene Symbol | DNAJC17 |
| Gene ID (NCBI) | 55192 |
| RRID | AB_3670044 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NVM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DnaJ proteins, also known as Hsp40, are a set of highly conserved proteins typically acting as co-factors of Hsp70 molecular chaperones. Based on their domain architecture, DNAJ is classified into three different groups (A, B, and C). DNAJC17 belongs to group C, bearing the J domain at its N-terminal region and an RNA recognition motif (RRM) located at the protein C-terminu. DNAJC17 is a susceptibility gene for congenital hypothyroidism and demonstrated its wide expression in mice since early embryogenesis, in which it plays an essential role in pre-implantation stages (PMID: 29773831).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC17 antibody 31580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |