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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJC15 Polyclonal antibody

DNAJC15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16063-1-AP

Synonyms

DNAJC15, DNAJD1, HSD18, MCJ

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 16063-1-AP

    WB analysis of mouse liver using 16063-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16063-1-AP (DNAJC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 16063-1-AP

    WB analysis of human brain using 16063-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16063-1-AP (DNAJC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16063-1-AP

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16063-1-AP

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16063-1-AP

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16063-1-AP

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16063-1-AP targets DNAJC15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC15 fusion protein Ag8911 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 15
Calculated molecular weight 150 aa, 16 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC010910
Gene symbol DNAJC15
Gene ID (NCBI) 29103
RRIDAB_10598307
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJC15 antibody 16063-1-APDownload protocol
IHC protocol for DNAJC15 antibody 16063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article