Validation Data Gallery

Filter: AllWBIHC

WB analysis using 17331-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17331-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17331-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17331-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 17331-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 17331-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 17331-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17331-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 17331-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

DNAJC11 Polyclonal antibody

DNAJC11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17331-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DNAJC11

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, SH-SY5Y cells, HepG2 cells, mouse liver tissue
Positive IHC detected in	human liver tissue, human skeletal muscle tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

17331-1-AP targets DNAJC11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11310 Product name: Recombinant human DNAJC11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-507 aa of BC014145 Sequence: MHISQSIEAPLTATDTAILSGSLSTQNGNGGGSINFALRRVTSAKGWGELEFGAGDLQGPLFGLKLFRNLTPRCFVTTNCALQFSSRGIRPGLTTVLARNLDKNTVGYLQWRWGIQSAMNTSIVRDTKTSHFTVALQLGIPHSFALISYQHKFQDDDQTRVKGSLKAGFFGTVVEYGAERKISRHSVLGAAVSVGVPQGVSLKVKELEKQRESAATDVLQKKQEAESAVRLMQESVRRIIEAEESRMGLIIVNAWYGKFVNDKSRKSEKVKVIDVTVPLQCLVKDSKLILTEASKAGLPGFYDPCVGEEKNLKVLYQFRGVLHQVMVLDSEALRIPKQSHRIDTDG 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily C, member 11
Calculated molecular weight	507 aa, 57 kDa
Observed molecular weight	63 kDa
GenBank accession number	BC014145
Gene Symbol	DNAJC11
Gene ID (NCBI)	55735
RRID	AB_2878385
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NVH1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.

Protocols

Product Specific Protocols
IHC protocol for DNAJC11 antibody 17331-1-AP	Download protocol
WB protocol for DNAJC11 antibody 17331-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution Authors - Michael J McKenna View Article
human	WB	J Proteome Res Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice. Authors - Foteini Violitzi View Article
mouse	WB	PLoS One A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice. Authors - Fotis Ioakeimidis View Article
human,mouse	WB	Nat Cell Biol Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space Authors - Elias Adriaenssens View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17331-1-AP

WB analysis of HeLa using 17331-1-AP

WB analysis of HeLa using 17331-1-AP

WB analysis of SH-SY5Y using 17331-1-AP

IHC staining of human skeletal muscle using 17331-1-AP

IHC staining of human liver using 17331-1-AP