Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HSC-T6 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells |
| Positive IHC detected in | human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66587-1-Ig targets DNAJB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2258 Product name: Recombinant human DNAJB6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC000177 Sequence: MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 6 |
| Calculated molecular weight | 241 aa, 27 kDa |
| Observed molecular weight | 27 kDa, 38 kDa |
| GenBank accession number | BC000177 |
| Gene Symbol | DNAJB6 |
| Gene ID (NCBI) | 10049 |
| RRID | AB_2881947 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75190 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| IHC protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| WB protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC | ||
Biomedicines Abnormally High Expression of DNAJB6 Accelerates Malignant Progression of Lung Adenocarcinoma
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