• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

DNAJB3 Polyclonal antibody

DNAJB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17177-1-AP

Synonyms

DNAJB3, HCG3

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 17177-1-AP

    WB analysis of mouse testis using 17177-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17177-1-AP

    WB analysis using 17177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17177-1-AP

    WB analysis using 17177-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 17177-1-AP

    WB analysis of rat testis using 17177-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 17177-1-AP

    IHC staining of mouse testis using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17177-1-AP

    IHC staining of mouse testis using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 17177-1-AP

    IHC staining of human kidney using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 17177-1-AP

    IHC staining of human kidney using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary using 17177-1-AP

    IHC staining of human ovary using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 17177-1-AP

    IHC staining of human ovary using 17177-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB3 fusion protein Ag10878 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 3
Calculated molecular weight 145 aa, 17 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC024013
Gene symbol DNAJB3
Gene ID (NCBI) 414061
RRIDAB_2094420
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB3 antibody 17177-1-APDownload protocol
IHC protocol for DNAJB3 antibody 17177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

PMID: 26400768

Authors - Mohamed Abu-Farha
View Article
mouseWB

Sci Rep

Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.

Authors - Abdoulaye Diane
  • KD Validated
View Article
humanWB,IHC

PLoS One

DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.

Authors - Abubaker Jehad J
View Article
mouseWB

Int J Mol Sci

Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.

Authors - Ryo Mitsugi
View Article
humanWB,IHC

Lipids Health Dis

Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.

Authors - Ali Tiss
View Article
mouseWB

Sci Rep

DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.

Authors - Abdelilah Arredouani
  • KD Validated
View Article