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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using DNAJB2 Monoclonal antibody (60204-1-Ig)

    WB analysis of SH-SY5Y using 60204-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60204-1-Ig (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DNAJB2 Monoclonal antibody

DNAJB2 Monoclonal Antibody for WB, ELISA
Cat No. 60204-1-Ig
Clone No.2G8C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60204-1-Ig targets DNAJB2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DNAJB2 fusion protein Ag1271 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 2
Calculated molecular weight 36 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC011609
Gene Symbol DNAJB2
Gene ID (NCBI) 3300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25686
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.