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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB1 Recombinant antibody, PBS Only

DNAJB1 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K3

Cat no : 82676-1-PBS

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1

Validation Data Gallery

Applications:
  • WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

    WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82676-1-RR (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

    IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

    IP result of anti-DNAJB1 (IP:82676-1-RR, 4ug; Detection:82676-1-RR 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJB1 antibody included
  • IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJB1 antibody included
  • IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

    IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (82676-1-RR, Clone: 4K3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

82676-1-PBS targets DNAJB1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.