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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB1 Recombinant antibody (82676-1-RR)

    WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82676-1-RR (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DNAJB1 Recombinant antibody (82676-1-RR)

    IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

    IP result of anti-DNAJB1 (IP:82676-1-RR, 4ug; Detection:82676-1-RR 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJB1 Recombinant antibody (82676-1-RR)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJB1 Recombinant antibody (82676-1-RR)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJB1 Recombinant antibody (82676-1-RR)

    IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (82676-1-RR, Clone: 4K3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82676-1-RR

    Affinity and Kinetic Characterization of 82676-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82676-1-RR against Human DNAJB1 were performed. The affinity constant is 0.213 nM.

DNAJB1 Recombinant monoclonal antibody, PBS Only

DNAJB1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82676-1-PBS
Clone No.4K3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82676-1-PBS targets DNAJB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3830

Product name: Recombinant human DNAJB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC019827

Sequence: MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene Symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25685
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

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