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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using DNAJA2 Polyclonal antibody (12236-1-AP)

    WB analysis of A375 using 12236-1-AP (same clone as 12236-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using DNAJA2 Polyclonal antibody (12236-1-AP)

    WB analysis of mouse kidney using 12236-1-AP (same clone as 12236-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using DNAJA2 Polyclonal antibody (12236-1-AP)

    WB analysis of human brain using 12236-1-AP (same clone as 12236-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12236-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A375 cells using DNAJA2 Polyclonal antibody (12236-1-AP)

    IP experiment of A375 using 12236-1-AP (same clone as 12236-1-PBS)

    IP result of anti-DNAJA2 (IP:12236-1-AP, 4ug; Detection:12236-1-AP 1:300) with A375 cells lysate 3600 ug. This data was developed using the same antibody clone with 12236-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DNAJA2 Polyclonal antibody (12236-1-AP)

    IF Staining of MCF-7 using 12236-1-AP (same clone as 12236-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12236-1-AP (DNAJA2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12236-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12236-1-PBS targets DNAJA2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJA2 fusion protein Ag2877 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily A, member 2
Calculated molecular weight 417 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC015809
Gene Symbol DNAJA2
Gene ID (NCBI) 10294
RRIDAB_2230709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60884
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.