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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJA1 Monoclonal antibody (67184-1-Ig)

    WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DNAJA1 Monoclonal antibody (67184-1-Ig)

    WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

    WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DNAJA1 Monoclonal antibody, PBS Only

DNAJA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67184-1-PBS
Clone No.1B10A10

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67184-1-PBS targets DNAJA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DNAJA1 fusion protein Ag2284 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Calculated molecular weight 397 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC008182
Gene Symbol DNAJA1
Gene ID (NCBI) 3301
RRIDAB_2882480
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDP31689
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.