Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human brain tissue, rat testis tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 4 publications below |
Product Information
12756-1-AP targets DNAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3473 Product name: Recombinant human DNAI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-699 aa of BC030583 Sequence: VGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAIYNLKKPHSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLKVEGSTTEVPEGLQLHQVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSWNPYHTKVFMSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKYEAICNQPVAAKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVREVKIKT 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, axonemal, intermediate chain 1 |
| Calculated molecular weight | 699 aa, 79 kDa |
| Observed molecular weight | 80-85 kDa |
| GenBank accession number | BC030583 |
| Gene Symbol | DNAI1 |
| Gene ID (NCBI) | 27019 |
| RRID | AB_10643244 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI46 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAI1 antibody 12756-1-AP | Download protocol |
| WB protocol for DNAI1 antibody 12756-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. | ||
Elife DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice | ||
J Hum Genet Novel variants in DNAH9 are present in two infertile patients with severe asthenospermia | ||
EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men |
