Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 14 publications below |
| CoIP | See 1 publications below |
Product Information
24488-1-AP targets DNAH17 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20033 Product name: Recombinant human DNAH17 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 299-644 aa of BC112284 Sequence: MEQADFTMLPTFIAKVLDTICFIWATSEYYNTPARIIVILQEFCNQIIEMTRTFLSPEEVLKGLQGEIEEVLSGISLAVNVLKELYQTYDFCCVNMKLFFKDKEPVPWEFPSSLAFSRINSFFQRIQTIEELYKTAIEFLKLEKIELGGVRGNLLGSLVTRIYDEVFELVKVFADCKYDPLDPGDSNFDRDYADFEIKIQDLDRRLATIFCQGFDDCSCIKSSAKLLYMCGGLMERPLILAEVAPRYSVMLELFDAELDNAKILYDAQMAASEEGNIPLIHKNMPPVAGQLKWSLELQERLEVSMKHLKHVEHPVMSGAEAKLTYQKYDEMMELLRCHREKIYQQW 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, axonemal, heavy chain 17 |
| Calculated molecular weight | 4485 aa, 512 kDa |
| Observed molecular weight | 500-512 kDa |
| GenBank accession number | BC112284 |
| Gene Symbol | DNAH17 |
| Gene ID (NCBI) | 8632 |
| RRID | AB_2879568 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UFH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNAH17 antibody 24488-1-AP | Download protocol |
| WB protocol for DNAH17 antibody 24488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility | ||
Nat Commun Doublet microtubule-associated tektins and enzymes differentially regulate sperm flagellar integrity and motility. | ||
Ecotoxicol Environ Saf Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway | ||
Reprod Biomed Online A novel mutation in DNAH17 is present in a patient with multiple morphological abnormalities of the flagella. | ||
Andrology A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans | ||
Hum Genet Biallelic loss-of-function variants of DNAH7 cause male infertility associated with asthenozoospermia in humans. |