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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using DNA2 Polyclonal antibody (18727-1-AP)

    WB analysis of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using DNA2 Polyclonal antibody (18727-1-AP)

    WB analysis of mouse thymus using 18727-1-AP (same clone as 18727-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using DNA2 Polyclonal antibody (18727-1-AP)

    WB analysis of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using DNA2 Polyclonal antibody (18727-1-AP)

    IP experiment of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

    IP result of anti-DNA2 (IP:18727-1-AP, 4ug; Detection:18727-1-AP 1:300) with mouse liver tissue lysate 4800 ug. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DNA2 Polyclonal antibody (18727-1-AP)

    IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DNA2 Polyclonal antibody (18727-1-AP)

    IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DNA2 Polyclonal antibody (18727-1-AP)

    IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNA2 Polyclonal antibody (18727-1-AP)

    IF Staining of HepG2 using 18727-1-AP (same clone as 18727-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA2 antibody (18727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DNA2 Polyclonal antibody, PBS Only

DNA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18727-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DNA2L, KIAA0083

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18727-1-PBS targets DNA2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name DNA replication helicase 2 homolog (yeast)
Calculated molecular weight 120 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_001080449
Gene Symbol DNA2
Gene ID (NCBI) 1763
RRIDAB_10637998
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51530
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

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