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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    IHC staining of human ovary tumor using 85855-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    IHC staining of human tonsillitis using 85855-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    IHC staining of human placenta using 85855-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    IF Staining of HeLa using 85855-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    IF Staining of HepG2 using 85855-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red).

  • Flow cytometry (FC) experiment of HEK-293T cells using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    FC experiment of HEK-293T using 85855-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.2 ug N/a DNA-RNA Hybrid Recombinant Antibody (85855-1-RR, Clone:250863A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Dot Blot experiment of / using DNA-RNA Hybrid Recombinant monoclonal antibody (85855-1-RR)

    Dot Blot experiment of / using 85855-1-RR

    Dot blot analysis was used to confirm the specificity of 85855-1-RR DNA-RNA Hybrid antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ng) spotted is indicated next to each row.

DNA-RNA Hybrid Recombinant monoclonal antibody

DNA-RNA Hybrid Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot blot, ELISA
Cat No. 85855-1-RR
Clone No.250863A1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, IF/ICC, FC (Intra), Dot blot, ELISA

Synonyms

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman placenta tissue, human ovary tumor tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells
Positive Dot blot detected in/ /

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85855-1-RR targets DNA-RNA Hybrid in IHC, IF/ICC, FC (Intra), Dot blot, ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

unknow

相同性解析による交差性が予測される生物種
Full Name DNA-RNA Hybrid
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA-RNA Hybrid antibodies are primarily used to study R-loops. Theoretically, they do not bind to single-stranded DNA, double-stranded DNA, or RNA. R-loops are DNA-RNA hybrid structures formed during transcription and are closely related to genome stability, DNA replication, DNA repair, and cancer development.

Protocols

Product Specific Protocols
IF protocol for DNA-RNA Hybrid antibody 85855-1-RRDownload protocol
IHC protocol for DNA-RNA Hybrid antibody 85855-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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