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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using DMKN Polyclonal antibody (16252-1-AP)

    WB analysis of mouse ovary using 16252-1-AP (same clone as 16252-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DMKN Polyclonal antibody (16252-1-AP)

    WB analysis using 16252-1-AP (same clone as 16252-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16252-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DMKN Polyclonal antibody (16252-1-AP)

    IP experiment of mouse lung using 16252-1-AP (same clone as 16252-1-PBS)

    IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 16252-1-PBS in a different storage buffer formulation.

DMKN Polyclonal antibody, PBS Only

DMKN Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16252-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16252-1-PBS targets DMKN in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9242

Product name: Recombinant human DMKN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-476 aa of BC011886

Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW

相同性解析による交差性が予測される生物種
Full Name dermokine
Calculated molecular weight 476 aa, 47 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC011886
Gene Symbol DMKN
Gene ID (NCBI) 93099
RRIDAB_10596473
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6E0U4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

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