Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 24813-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24813-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 24813-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 24813-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 24813-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 24813-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 24813-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DMGDH Polyclonal antibody

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 24813-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

24813-1-AP targets DMGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DMGDH fusion protein Ag20529 相同性解析による交差性が予測される生物種
Full Name	dimethylglycine dehydrogenase
Calculated molecular weight	866 aa, 97 kDa
Observed molecular weight	97 kDa
GenBank accession number	BC022388
Gene Symbol	DMGDH
Gene ID (NCBI)	29958
RRID	AB_2879739
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UI17
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.

Protocols

Product Specific Protocols
WB protocol for DMGDH antibody 24813-1-AP	Download protocol
IHC protocol for DMGDH antibody 24813-1-AP	Download protocol
IF protocol for DMGDH antibody 24813-1-AP	Download protocol
FC protocol for DMGDH antibody 24813-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. Authors - I-Ling Lu View Article
human	WB,IHC	Oncotarget Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo. Authors - Gang Liu View Article
human	WB	J Proteome Res Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion. Authors - Yin Cao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 24813-1-AP

WB analysis of mouse liver using 24813-1-AP

WB analysis of rat liver using 24813-1-AP

IHC staining of human liver cancer using 24813-1-AP

IHC staining of human liver cancer using 24813-1-AP

IF Staining of HepG2 using 24813-1-AP

FC experiment of HepG2 using 24813-1-AP