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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMAP1 Monoclonal antibody (67183-1-Ig)

    WB analysis using 67183-1-Ig (same clone as 67183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67183-1-Ig (DMAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DMAP1 Monoclonal antibody (67183-1-Ig)

    IF Staining of HEK-293 using 67183-1-Ig (same clone as 67183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DMAP1 antibody (67183-1-Ig, Clone: 1A4E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67183-1-PBS in a different storage buffer formulation.

DMAP1 Monoclonal antibody, PBS Only

DMAP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67183-1-PBS
Clone No.1A4E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67183-1-PBS targets DMAP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28906

Product name: Recombinant human DMAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-467 aa of BC002855

Sequence: MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYALLYSDKKDAPPLLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEEGKDYPFARFNKTVQVPVYSEQEYQLYLHDDAWTKAETDHLFDLSRRFDLRFVVIHDRYDHQQFKKRSVEDLKERYYHICAKLANVRAVPGTDLKIPVFDAGHERRRKEQLERLYNRTPEQVAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAEQRRTERKAPKKKLPQKKEAEKPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGVELSPTPTEELVHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGGPATPASGPGPASAEPAVTEPGLGPDPKDTIIDVVGAPLTPNSRKRRESASSSSSVKKAKKP

相同性解析による交差性が予測される生物種
Full Name DNA methyltransferase 1 associated protein 1
Calculated molecular weight 53 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC002855
Gene Symbol DMAP1
Gene ID (NCBI) 55929
RRIDAB_2882479
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NPF5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).