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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLX6 Recombinant antibody (85946-1-RR)

    WB analysis using 85946-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85946-1-RR (DLX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DLX6 Recombinant antibody (85946-1-RR)

    IHC staining of mouse embryo using 85946-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DLX6 Recombinant antibody (85946-1-RR)

    IHC staining of mouse embryo using 85946-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85946-1-RR

    Affinity and Kinetic Characterization of 85946-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85946-1-RR against Human DLX6 were performed. The affinity constant is 5.65 nM.

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DLX6 Recombinant antibody

DLX6 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85946-1-RR
Clone No.250408B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Homeobox protein DLX-6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85946-1-RR targets DLX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DLX6 fusion protein Ag19724 相同性解析による交差性が予測される生物種
Full Name distal-less homeobox 6
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight32 kDa
GenBank accession numberBC069363
Gene Symbol DLX6
Gene ID (NCBI) 1750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Distal-less homeobox 6 (DLX6) has been reported to play important roles in the development of craniofacial structures, inner ear, limb, and brain. DLX6 was significantly highly expressed in oral cancer tissues in The Cancer Genome Atlas database. DLX6 promotes cell proliferation and suppresses cell apoptosis in oral cancer cells. EGFR-CCND1 pathway might be the potential mechanism participating in the regulating axis (PMID: 33215805).

Protocols

Product Specific Protocols
WB protocol for DLX6 antibody 85946-1-RRDownload protocol
IHC protocol for DLX6 antibody 85946-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols