Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, A375 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
13046-1-AP targets DLX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DLX1 fusion protein Ag3586 相同性解析による交差性が予測される生物種 |
| Full Name | distal-less homeobox 1 |
| Calculated molecular weight | 255 aa, 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC036189 |
| Gene Symbol | DLX1 |
| Gene ID (NCBI) | 1745 |
| RRID | AB_2093117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Distal-less homeobox 1(DLX1) is a member of the DLX family of homeobox transcription factors, which is essential for the production of forebrain GABAergic interneurions during embryonic development. The DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6) is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX1 possible has a role in craniofacial patterning and morphogenesis and involves in the early development of diencephalic subdivisions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DLX1 antibody 13046-1-AP | Download protocol |
| IHC protocol for DLX1 antibody 13046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Cullin 4B-RING E3 ligase negatively regulates the immunosuppressive capacity of mesenchymal stem cells by suppressing iNOS |