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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLL4 Polyclonal antibody (21584-1-AP)

    WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using DLL4 Polyclonal antibody (21584-1-AP)

    WB analysis of mouse brain using 21584-1-AP (same clone as 21584-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DLL4 Polyclonal antibody (21584-1-AP)

    IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DLL4 Polyclonal antibody (21584-1-AP)

    IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DLL4 Polyclonal antibody (21584-1-AP)

    IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using DLL4 Polyclonal antibody (21584-1-AP)

    IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DLL4 Polyclonal antibody, PBS Only

DLL4 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 21584-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Delta4, hdelta2, UNQ1895/PRO4341

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21584-1-PBS targets DLL4 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16140

Product name: Recombinant human DLL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 317-530 aa of BC106950

Sequence: TGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLPPSFPWV

相同性解析による交差性が予測される生物種
Full Name delta-like 4 (Drosophila)
Calculated molecular weight 685 aa, 75 kDa
Observed molecular weight 70-100 kDa
GenBank accession numberBC106950
Gene Symbol DLL4
Gene ID (NCBI) 54567
RRIDAB_2878888
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.

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