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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLG5 Polyclonal antibody (15687-1-AP)

    WB analysis using 15687-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DLG5 Polyclonal antibody (15687-1-AP)

    WB analysis of PC-3 using 15687-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using DLG5 Polyclonal antibody (15687-1-AP)

    WB analysis of mouse kidney using 15687-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human bowens disease using DLG5 Polyclonal antibody (15687-1-AP)

    IHC staining of Human bowens disease using 15687-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human bowens disease using DLG5 Polyclonal antibody (15687-1-AP)

    IHC staining of Human bowens disease using 15687-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DLG5 Polyclonal antibody

DLG5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15687-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Placenta and prostate DLG, PDLG, KIAA0583, Disks large homolog 5, Discs large protein P-dlg

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, mouse kidney tissue, HeLa cells
Positive IHC detected inHuman bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15687-1-AP targets DLG5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLG5 fusion protein Ag8194 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 5 (Drosophila)
Calculated molecular weight 1893 aa, 209 kDa
Observed molecular weight 75 kDa, 240 kDa
GenBank accession numberBC002915
Gene Symbol DLG5
Gene ID (NCBI) 9231
RRIDAB_10642568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Discs large homolog 5 (DLG5) belongs to the membrane-associated guanylate kinase (MAGUK) family. DLG5 participates in epithelial cell polarity maintenance and cancer development. DLG5 is highly expressed in normal tissues, but its expression is decreased in many cancers, such as bladder cancer, prostate cancer, breast cancer, and hepatocellular carcinoma (PMID: 32015336). Down-regulation of DLG5 is highly correlated with clinical tumor stage. In breast cancer, knockdown of DLG5 induces cell migration, and overexpression of DLG5 inhibits cell migration (PMID: 28169360). DLG5 has 3 isoforms with the molecular weight of 75, 214 and 240 kDa.

Protocols

Product Specific Protocols
WB protocol for DLG5 antibody 15687-1-APDownload protocol
IHC protocol for DLG5 antibody 15687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.

Authors - Rui Su
View Article