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  • Featured Product
  • KD/KO Validated

DLEU1 Polyclonal antibody

DLEU1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11860-1-AP

Synonyms

DLB1, DLEU1, DLEU2, LEU1, LEU2, NCRNA00021, XTP6

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 11860-1-AP

    WB analysis of K-562 using 11860-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11860-1-AP (DLEU1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 11860-1-AP

    WB analysis of A549 using 11860-1-AP

    WB result of DLEU1 antibody (11860-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLEU1 transfected A549 cells.

  • WB analysis of K-562 using 11860-1-AP

    WB analysis of K-562 using 11860-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11860-1-AP (DLEU1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11860-1-AP

    IHC staining of human colon cancer using 11860-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11860-1-AP (DLEU1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 11860-1-AP

    IHC staining of human colon cancer using 11860-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11860-1-AP (DLEU1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11860-1-AP

    IHC staining of human lung cancer using 11860-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11860-1-AP (DLEU1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11860-1-AP

    IHC staining of human lung cancer using 11860-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11860-1-AP (DLEU1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

11860-1-AP targets DLEU1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLEU1 fusion protein Ag2435 相同性解析による交差性が予測される生物種
Full Name deleted in lymphocytic leukemia 1 (non-protein coding)
Calculated molecular weight 78 aa, 9 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC020692
Gene symbol DLEU1
Gene ID (NCBI) 10301
RRIDAB_874117
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DLEU1 antibody 11860-1-APDownload protocol
IHC protocol for DLEU1 antibody 11860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

The effects of DLEU1 gene expression in Burkitt lymphoma (BL): potential mechanism of chemoimmunotherapy resistance in BL.

Authors - Sanghoon Lee
View Article