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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of NIH/3T3 using 67702-1-Ig (same clone as 67702-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of HSC-T6 using 67702-1-Ig (same clone as 67702-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLD Monoclonal antibody (67702-1-Ig)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLD Monoclonal antibody (67702-1-Ig)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DLD Monoclonal antibody, PBS Only

DLD Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67702-1-PBS
Clone No.2E7G5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2E7G5, DLDH, EC:1.8.1.4, GCSL, LAD

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67702-1-PBS targets DLD in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9680

Product name: Recombinant human DLD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 160-509 aa of BC018696

Sequence: NQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

相同性解析による交差性が予測される生物種
Full Name dihydrolipoamide dehydrogenase
Calculated molecular weight 509 aa, 54 kDa
Observed molecular weight54 kDa
GenBank accession numberBC018696
Gene Symbol DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP09622
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

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