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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLC1 Monoclonal antibody (66894-1-Ig)

    WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DLC1 Monoclonal antibody (66894-1-Ig)

    WB analysis of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using DLC1 Monoclonal antibody (66894-1-Ig)

    IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLC1 Monoclonal antibody (66894-1-Ig)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLC1 Monoclonal antibody (66894-1-Ig)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

DLC1 Monoclonal antibody, PBS Only

DLC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66894-1-PBS
Clone No.1G10H12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66894-1-PBS targets DLC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24404

Product name: Recombinant human DLC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 111-370 aa of BC054511

Sequence: SDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQDEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIE

相同性解析による交差性が予測される生物種
Full Name deleted in liver cancer 1
Calculated molecular weight 171 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
RRIDAB_2882223
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ96QB1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.

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