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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLAT Recombinant monoclonal antibody (83654-3-RR)

    WB analysis using 83654-3-RR (same clone as 83654-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83654-3-RR (DLAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00635-4

    Sandwich ELISA sample test of MP00635-4

    The mean DLAT concentration was determined to be 115.60 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 96.07 ng/mL in HEK-293 cell extract based on a 2.3 mg/mL extract load.

  • Sandwich ELISA standard curve of MP00635-4

    Sandwich ELISA standard curve of MP00635-4

    Sandwich ELISA standard curve of MP00635-4, Human DLAT Recombinant Matched Antibody Pair - PBS only. 83654-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag22831. 83654-5-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DLAT Recombinant monoclonal antibody (83654-3-RR)

    IHC staining of human stomach cancer using 83654-3-RR (same clone as 83654-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83654-3-RR (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLAT Recombinant monoclonal antibody (83654-3-RR)

    IF Staining of HepG2 using 83654-3-RR (same clone as 83654-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (83654-3-RR, Clone: 240713C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using DLAT Recombinant monoclonal antibody (83654-3-RR)

    FC experiment of HepG2 using 83654-3-RR (same clone as 83654-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DLAT Recombinant Antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DLAT Recombinant monoclonal antibody (83654-3-RR)

    FC experiment of HeLa using 83654-3-RR (same clone as 83654-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DLAT Recombinant antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), or 0.25 ug DLAT Recombinant antibody (83654-3-RR, Clone: 240713C12) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83654-3-RR

    Affinity and Kinetic Characterization of 83654-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83654-3-RR against Human DLAT were performed. The affinity constant is 0.17 nM.

DLAT Recombinant monoclonal antibody, PBS Only (Capture)

DLAT Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 83654-3-PBS
Clone No.240713C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

240713C12, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83654-3-PBS targets DLAT as part of a matched antibody pair:

MP00635-4: 83654-3-PBS capture and 83654-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22831

Product name: Recombinant human DLAT protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-322 aa of BC039084

Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT

相同性解析による交差性が予測される生物種
Full Name dihydrolipoamide S-acetyltransferase
Calculated molecular weight 647 aa, 70 kDa
Observed molecular weight 69-70 kDa
GenBank accession numberBC039084
Gene Symbol DLAT
Gene ID (NCBI) 1737
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10515
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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