DLAT Monoclonal antibody
DLAT Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1F2B9
Cat no : 68303-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HCT 116 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:1500-1:6000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
68303-1-Ig targets DLAT in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DLAT fusion protein Ag4226 相同性解析による交差性が予測される生物種 |
| Full Name | dihydrolipoamide S-acetyltransferase |
| Calculated molecular weight | 647 aa, 70 kDa |
| Observed molecular weight | 69-70 kDa |
| GenBank accession number | BC039084 |
| Gene symbol | DLAT |
| Gene ID (NCBI) | 1737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DLAT antibody 68303-1-Ig | Download protocol |
| IHC protocol for DLAT antibody 68303-1-Ig | Download protocol |
| IF protocol for DLAT antibody 68303-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol The signature of cuproptosis-related immune genes predicts the tumor microenvironment and prognosis of prostate adenocarcinoma | ||
Acta Biomater Copper-Coordinated Nanoassemblies based on Photosensitizer-Chemo Prodrugs and Checkpoint Inhibitors for Enhanced Apoptosis-Cuproptosis and Immunotherapy |
