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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using DKK4 Polyclonal antibody (27080-1-AP)

    WB analysis of mouse cerebellum using 27080-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27080-1-AP (DKK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using DKK4 Polyclonal antibody (27080-1-AP)

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using DKK4 Polyclonal antibody (27080-1-AP)

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 40x lens).

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DKK4 Polyclonal antibody

DKK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27080-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27080-1-AP targets DKK4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK4 fusion protein Ag24209 相同性解析による交差性が予測される生物種
Full Name dickkopf homolog 4 (Xenopus laevis)
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC107047
Gene Symbol DKK4
Gene ID (NCBI) 27121
RRIDAB_2880746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DKK4 antibody 27080-1-APDownload protocol
IHC protocol for DKK4 antibody 27080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Clin Transl Med

O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signalling

Authors - Zhongqi Cui
View Article